Table 1.

Characteristics of the identified CalS genes and proteins from stinging nettle. The accession numbers, transcript and protein length, as well as the E-value, amino acid start/end and names of the respective conserved domains are provided together with the number of transmembrane domains predicted with TMHMM, Phobius and TOPCONS.

Gene Name with Accession Numbers	Transcript Length (nt)	Protein Length (aa)	Conserved Domains					Number of Transmembrane Domains
			From aa	To aa	E-Value	Conserved Protein Domain Family	Short Name	TMHMM	Phobius	TOPCONS
UdCalS1 MT468368	5886	1961	1045	1805	0	pfam02364	Glucan_synthase superfamily	16	17	19
			312	424	9.97 × 10−53	pfam14288	FKS1_dom1
			45	170	5.69 × 10−14	pfam04652	Vta1 superfamily
UdCalS3 MT468366	5886	1961	1059	1817	0	pfam02364	Glucan_synthase superfamily	17	18	17
			323	436	2.83 × 10−55	pfam14288	FKS1_dom1
			52	179	1.78 × 10−17	pfam04652	Vta1
UdCalS6 (partial) MT468373	3249	1082	216	961	0	pfam02364	Glucan_synthase superfamily	10	10	11
UdCalS7 MT468367	5778	1925	1042	1782	0	pfam02364	Glucan_synthase superfamily	15	17	20
			335	445	3.44 × 10−49	pfam14288	FKS1_dom1
			56	191	4.57 × 10−11	pfam04652	Vta1 superfamily
UdCalS8 MT468374	5913	1970	1102	1834	0	pfam02364	Glucan_synthase superfamily	13	17	17
			346	447	5.48 × 10−48	pfam14288	FKS1_dom1
			65	115	0.00046	pfam04652	Vta1 superfamily
UdCalS9 MT468369	5706	1901	1023	1766	0	pfam02364	Glucan_synthase superfamily	16	17	17
			337	446	2.45 × 10−54	pfam14288	FKS1_dom1
UdCalS10 MT468370	5712	1903	1026	1768	0	pfam02364	Glucan_synthase superfamily	15	15	18
			347	457	4.65 × 10−52	pfam14288	FKS1_dom1
UdCalS11 MT468372	5406	1801	897	1669	0	pfam02364	Glucan_synthase	13	17	19
			174	287	8.84 × 10−52	pfam14288	FKS1_dom1
UdCalS12 MT468371	5322	1773	875	1639	0	pfam02364	Glucan_synthase	12	18	19
			169	270	5.78 × 10−47	pfam14288	FKS1_dom1