Figure 2.

β-funaltrexamine alleviated neuronal cell death. Neuron/glia cultures were pretreated with vehicle or β-funaltrexamine (30 μM) for 30 min before being incubated with LPS (100 ng/mL)/IFN-γ (10 U/mL) for an additional 48 h. Cells were subjected to immunofluorescence staining with antibodies recognizing Microtubule-Associated Protein 2 (MAP-2), CD68, and Glial Fibrillary Acidic Protein (GFAP). The cell nuclei were counterstained with Hoechst 33342. Representative micrographs are shown. Bar = 60 μm (A). Quantitative numbers of immunopositivity are shown (B). Total cellular proteins were extracted and subjected to Western blot analysis with indicated antibodies. One representative blot of four independent culture batches is shown (C). Cell damage was measured by LDH efflux assay (D). * p < 0.05 vs. untreated control and # p < 0.05 vs. LPS/IFN-γ control, n = 4.