Figure 3.

β-funaltrexamine alleviated microglial activation. Neuron/glia cultures were pretreated with vehicle or β-funaltrexamine (30 μM) for 30 min before being incubated with LPS (100 ng/mL)/IFN-γ (10 U/mL) for an additional 24 h. (A) Total cellular proteins were extracted and subjected to Western blot analysis with indicated antibodies. One representative blot of four independent culture batches is shown. (B) Total cellular RNAs were extracted and subjected to quantitative RT-PCR for the measurement of CD163 and arginase 1 mRNA expression. * p < 0.05 vs. untreated control and # p < 0.05 vs. LPS/IFN-γ control, n = 4.