Figure 5.

Quantitative data of β-funaltrexamine-alleviated intracellular signaling molecules: Neuron/glia cultures were pretreated with vehicle or β-funaltrexamine (30 μM) for 30 min before being incubated with LPS (100 ng/mL)/IFN-γ (10 U/mL) for an additional 4 h. (A) Total cellular proteins were extracted and subjected to Western blot analysis with indicated antibodies. (B) Nuclear proteins were extracted and subjected to EMSA for the measurement of NF-κB, AP-1, CREB, and Stat DNA binding activities. Quantitative data are shown. * p < 0.05 vs. untreated control and # p < 0.05 vs. LPS/IFN-γ control, n = 4.