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Histopathology and immunohistochemical analyses in the mouse model of cross-reactive metal allergy. At 7 days after the third challenge, Hematoxylin and Eosin (H&E) staining showed epithelial acanthosis, as well as epidermal spongiosis and liquefaction degeneration (arrows) in Ni-Pd and Cr-Pd groups. CD3+ T cells were also present in the epithelial basal layer and the upper dermis in the Ni-Pd and Cr-Pd groups. Scale bar = 10 µm.
