Figure 7.

Schedule of the sensitization and challenge of the metal allergy cross-reactive mouse model. Sensitization using NiCl₂ + lipopolysaccharide (LPS) or CrCl₂ + LPS or saline were injected twice (at an interval of 7 days) intradermally (i.d.) into the left and right groin of mice. At 7 days after sensitization, mice were challenged for the first time. PdCl₂ was injected into the left and right footpad by i.d. injection to challenge and elicit an immune reaction. Challenge was repeated three times at an interval of 14 days.