Figure 4.

Vector designs and protein production of Int_C-Fc. (a) Primary structure of the hinge region of human IgG. (b) Vector maps of three constructs (Int_C-Fc, Int_C+1S-Fc, and Int_C-CPPC-Fc) in the backbone of pNCM02 and pNY326 vector. (c) Optimization of the growth media. Production of Int_C-Fc was compared in TM and SY media. Lanes 2d, 3d, 4d indicate SDS-PAGE analysis of the culture supernatant after 2 days, 3 days, and 4 days of protein expression, respectively. (d) Comparison of protein production between Int_C-Fc, Int_C+1S-Fc, and Int_C-CPPC-Fc by SDS-PAGE analysis. Lanes S and P indicate supernatant and pellet fractions from the culture medium after four days of protein expression, respectively. The control cell wall protein (HWP) and Int_C-Fc are marked with arrows.