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. 2020 Jun 3;21(11):4011. doi: 10.3390/ijms21114011

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A lectin fusion protein, ‘lectibody’ (a) Schematic drawing of the ligation of a lectin (SVN) with a model protein (GB1) by PTS. (b) SDS-PAGE analysis of the protein ligation of SVN and GB1. The first two lanes show separately prepared N- and C-precursor proteins (TRX-H₆-SVN-Int_N and Int_C-GB1). Lanes 3 and 4 show the ligation reaction immediately after the mixture and after 8 h of incubation, respectively. Lane 5 and 6 indicate samples before and after the proteolytic digestion by enterokinase (EK), respectively. Lanes 7 and 8 show samples before loading on a protein A sepharose column and the elution fraction from the column, respectively. (c) [¹H,¹⁵N]-HSQC spectrum of the segmentally isotopic labeled [¹⁵N]-SVN-[¹⁴N]-GB. (d) Schematic drawing of the production of SVN-Fc fusion by PTS. (e) SDS-PAGE analysis of the time-course of the ligation between TRX-H₆-SVN-Int_N and Int_C-CPPC-Fc by PTS.