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. 2020 Jun 24;20:272. doi: 10.1186/s12935-020-01369-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — LINC00242 interacts with miR-141 and positively regulats FOXC1, a target gene of miR-141. a The predicted localization of LINC00242. The abscissa represents different cell lines and the ordinate represents the localization of LINC00242; the histogram above the 0 line represents the expression mainly in the cytoplasm, and the histogram below the 0 line represents the expression mainly in the nucleus. b A heat map illustrating differentially expressed miRNAs from GC-related GSE26595 dataset. c The intersecting miRNAs from the GSE26595 dataset and the RAID database (http://www.rna-society.org/raid/search.html). d A heat map illustrating some significantly upregulated genes from the GSE13861 dataset. e The intersecting genes from the mirDIP (http://ophid.utoronto.ca/mirDIP/index.jsp#r), TargetScan (http://www.targetscan.org/vert_71/) and DIANA (http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=microT_CDS/index) databases and the GSE13861 dataset. f Subcellular localization of LINC00242 detected by FISH assay (× 400, scale bar = 25 μm). Green represents LINC00242 expression and blue represents the stained nucleus. g The binding of LINC00242 and FOXC1 to AGO2 assessed by RIP assay. * indicates p < 0.05 compared with IgG. h Putative miR-141 binding sites in the FOXC1 3′UTR by a web-available prediction (www.targetscan.org). i The luciferase activity at the promoter of the reporter gene containing FOXC1-WT and FOXC1-MUT upon miR-141 mimic transfection. * indicates p < 0.05 compared with mimic NC. j Expression of FOXC1 binding to using anti-AGO2 antibody in upon LINC00242 knockdown detected by RIP assay. * indicates p < 0.05 compared with si-NC. k Expression of miR-141 and FOXC1 in cells upon si-LINC00242 transfection detected by RT-qPCR, normalized to U6 and GAPDH respectively. * indicates p < 0.05 compared with si-NC. l Expression of FOXC1 in cells transfected with miR-141 mimic detected by RT-qPCR, normalized to GAPDH. * indicates p < 0.05 compared with mimic NC. m Expression of FOXC1 in cells transfected with si-LINC00242 or miR-141 inhibitor detected by RT-qPCR, normalized to GAPDH. Results are presented as mean ± S.D. of three technical replicates. * (compared with si-NC + inhibitor NC) and # (compared with si-LINC00242 + inhibitor NC) indicate p < 0.05 by unpaired t-test or one-way ANOVA followed by Tukey’s test