Skip to main content
. Author manuscript; available in PMC: 2020 Jun 24.
Published in final edited form as: Cell Rep. 2020 Jun 2;31(9):107705. doi: 10.1016/j.celrep.2020.107705

Figure 3. APOBEC3A-Induced Abasic Sites Slow Replication Elongation.

Figure 3.

(A) Three clones (c2, c9, and c11) of HCT116 GFP-APOBEC3A-ERT2 cells, or parental HCT116 cells, were incubated with 4-OHT, CldU, and IdU, as indicated, and processed for DNA combing.

(B) The uracil-DNA glycosylase inhibitor (UGI) or an empty vector (EV) was introduced by lentiviral infection before DNA combing.

(C) hTERT-RPE-1 cells were harvested and lysed 16 h after UGI was introduced by lentiviral infection to assess UNG inhibition.

(D) hTERT-RPE-1 GFP-APOBEC3A-ERT2 cells were infected with EV or UGI before DNA combing. All bars represent the median, and p values were derived from an ANOVA with Dunn’s multiple comparisons post-test.