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. Author manuscript; available in PMC: 2020 Jun 24.
Published in final edited form as: Cell Rep. 2020 Jun 2;31(9):107705. doi: 10.1016/j.celrep.2020.107705

Figure 4. HMCES and TLS Slow Replication Elongation in Response to Nuclear APOBEC3A.

Figure 4.

(A) Cells were transfected with HMCES (H-1 and H-2), POLK, or a non-targeting siRNA (NT) and were incubated with 4-OHT before DNA combing.

(B) UGI or empty vector (EV) was introduced by lentiviral infection.

(C) Cells were transfected with HMCES or non-targeting siRNA, treated with DMSO, 4-OHT, and 10 μM JH-RE-06 for 30 min as indicated, and processed for DNA combing.

(D) Cells were transfected with HMCES and POLK siRNA, treated with 4-OHT, and processed for DNA combing. Bars represent the median, and p values were derived from an ANOVA with Dunn’s multiple comparisons post-test.