| Antibodies |
|
|
| Rabbit polyclonal anti HMCES |
Sigma |
Cat#HPA044968; RRID:AB_2679160 |
| Rabbit polyclonal anti APOBEC3A |
Sigma |
Cat# SAB4500753; RRID:AB_10742606 |
| Rabbit polyclonal anti GFP |
Abcam |
Cat#ab290; RRID:AB_303395 |
| Mouse monoclonal anti BrdU |
BD |
Cat#347580; RRID:AB_10015219 |
| Rat monoclonal anti BrdU |
Abcam |
Cat#ab6326; RRID:AB_305426 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 |
Life Technologies |
Cat# A32723; RRID:AB_2633275 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Life Technologies |
Cat# A11007; RRID:AB_10561522 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| JHRE06 |
VICB synthesis core |
N/A |
| CldU |
Sigma Aldrich |
Cat#C6891 |
| IdU |
Sigma Aldrich |
Cat#l7125 |
| EdU |
VICB synthesis core |
N/A |
| 4-Hydroxytamoxifen |
Tocris |
Cat#3412/10 |
| MG-132 |
Sigma Aldrich |
Cat#M7449 |
| Hoescht 33342 |
ThermoFisher |
Cat#62249 |
| Alexa Fluor 488 Azide |
ThermoFisher |
Cat#A10266 |
| APE-I |
NEB |
Cat#M0282S |
| Uracil-DNA Glycosylase (UDG) |
NEB |
Cat#M0280S |
| Critical Commercial Assays |
|
|
| Duolink In Situ PLA Probe Anti-Rabbit PLUS |
Sigma-Aldrich |
Cat#DU092002
|
| Duolink In Situ PLA Probe Anti-Rabbit MINUS |
Sigma-Aldrich |
Cat#DU092004
|
| Duolink In Situ Detection Reagents, Red |
Sigma-Aldrich |
Cat#DU092008
|
| Comet Assay Kit |
Trevigen |
Cat#4250–050-K |
| FiberPrep Kit |
Genomic Vision |
Cat#EXTR-001 |
| Combi Coverslips |
Genomic Vision |
Cat#COV-002-RUO |
| U2OS |
ATCC |
Cat#HTB-96; RRID:CVCL_0042 |
| hTERT-RPE-1 |
ATCC |
Cat#CRL-4000; RRID:CVCL_4388 |
| HCT-116 |
ATCC |
Cat#CCL-247; RRID:CVCL_0291 |
| Oligonucleotides |
|
|
| HMCES siRNA |
Dharmacon |
Cat#D-020333–01 |
| HMCES siRNA |
Dharmacon |
Cat#J-020333–19 |
| PolK siRNA |
Dharmacon |
Cat#D-021038–01 |
| PolK siRNA |
Dharmacon |
Cat#D-021038–02 |
| PolK siRNA |
Dharmacon |
Cat#D-021038–03 |
| PolK siRNA |
Dharmacon |
Cat#D-021038–04 |
| APEX1 siRNA |
Dharmacon |
Cat#D-010237–01 |
| APEX1 siRNA |
Dharmacon |
Cat#D-010237–02 |
| APEX1 siRNA |
Dharmacon |
Cat#D-010237–03 |
| APEX1 siRNA |
Dharmacon |
Cat#D-010237–05 |
| APEX2 siRNA |
Dharmacon |
Cat#D-013730–01 |
| APEX2 siRNA |
Dharmacon |
Cat#D-013730–02 |
| APEX2 siRNA |
Dharmacon |
Cat#D-013730–03 |
| APEX2 siRNA |
Dharmacon |
Cat#D-013730–05 |
| AllStars Negative Control siRNA |
QIAGEN |
Cat#1027281 |
| Recombinant DNA |
|
|
| pAcGFP-ERT2 |
Haahr et al., 2016 |
Mailand Lab |
| APOBEC3A cDNA |
Dharmacon |
Cat# OHS5894–9916829 |
| pAcGFP-APOBEC3A-ERT2 |
This paper |
N/A |
| PiggyBac Transposon Vectors |
Systems Biosciences |
Cat# PB510B-1 |
| PiggyBac-GFP-APOBEC3A-ERT2 |
This paper |
N/A |
| Software and Algorithms |
|
|
| Graphpad Prism |
Graphpad Software |
https://www.graphpad.com/scientific-software/prism/; RRID: SCR_000306 |
| Fiji |
Schindelin et al., 2012 |
http://fiji.sc RRID:SCR_002285 |
| OpenComet |
Gyori et al., 2014 |
N/A |
| Other |
|
|
