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. 2020 Mar 27;103(1):24–35. doi: 10.1093/biolre/ioaa036

Figure 5.

Figure 5

Premature homolog disjunction in Chtf18−/− oocytes during prophase I. Meiotic chromosome spreads from wild-type and Chtf18−/− oocytes were prepared and immunostained with SYCP3 antibody (synaptonemal complex, green) and CREST autoimmune serum (centromeres, gray or red). CREST foci were scored during the pachytene (A) and diplotene (B) stages of prophase I. Although Chtf18−/− and wild-type oocytes contained 20 CREST foci per nucleus during the pachytene stage (n = 10 embryos per genotype, P = 0.4), Chtf18−/− oocytes contained significantly greater foci per nucleus during the diplotene stage. Scale bars, 10 μm. (C) Bar graph depicting CREST foci per nucleus during diplonema. Values are the mean ± SEM (wild-type n = 8 embryos; Chtf18−/−n = 13 embryos; P = 0.0003***, unpaired t-test with Welch’s correction).