Table 1.

Primer pairs and FRET probes sequences used for real time PCR gene expression analyses.

Gene		Sequences of primers (F, R) and probes (FRET—FL, LC, hydrolysis probes—TM)	Length (bp)
PLIN2 Gene ID: 397402	F	AGCTAGAGCCTCAGATTGCCAT	246
	R	GAATGCTTTTTCTACTCCACTGCTC
	FL	AAGACCAAGTCTGTGGTTAATGGAAGCA
	LC	ACACTGTCCTGGGAAGTCGGATGATG
FADS1 Gene ID: 444995	F	CAAGAGGAAGAAGTGGGTGGACT	210
	R	CATGGAGAACCAGACCACGTT
	FL	CAACAGTGGCACATAAGTGAGGAAGATGC
	LC	CACATAGAAGGACGTCATCCAGGCCA
ACACA Gene ID: 397324	F	CCTGGATTCTGAAGCCAAGATAATC	206
	R	CGTAAGCCGTCCACGATGTAA
	FL	CCCTTCGCGGTTGAAGTCCTTGA
	LC	GCCTGATAGGTCTTAAACGCAGAGTCTGGA
SCD Gene ID: 396670	F	GGTGATGTTCCAGAGGAGGTACT	241
	R	CCAATGAAACCAGGATATTCTCC
	FL	GCCATCGTGCTCAATGCCACCT
	LC	CTGGTAAACAGTGCTGCCCACCTATACG
FASN Gene ID: 397561	F	CCTCACCTCCATCCAGATC	149
	R	CAGTAGCTGGAGAGCACAG
	FL	CCACCTCGCCCAGGGAGT
	LC	CCCGATGATGCCGTCAGGCT
ACTB Gene ID: 414396	F	CAAAGCCAACCGTGAGAAGA	121
	R	GTACCCCTCGTAGATGGGCA
	FL	TGTCCCTGTACGCCTCTGGCC
	LC	CACCACTGGCATCGTGATGGACTCCG
GAPDH Gene ID: 396823	F	CCCACGAGCACACCTCAGAA	141
	R	TGCAGCCTGTACTCCCGCT
	FL	GGCAGCAGGTGGGACAGCAG
	LC	GGGTGCAGGAGAGGGGCGAC
SREBF1 Gene ID: 397308	F	CACACCATGGGGAAGTACC	134
	R	GGAGACTGGTCTTGACTCGC
	TM	F-CAGCACGGCCACGGACAGG-Q
ATGL Gene ID: 100049704	F	GGGTCTGCCTGGGTGATAC	236
	R	GACGTTGGCCTGGATGAG
	TM	F-CACTGCACCCCTCCTTCAACCTG-Q
PPARG Gene ID: 397671	F	CACTGGAATTAGATGACAGCGAC	87
	R	TGTCTTGAATGTCCTCGATGG
	TM	F-CGCCCAGGTTTGCTGAATGT-Q
FADS2 Gene ID: 444997	F	GACGGCCTTCATCCTTGCTA	375
	R	CTGGTACTGGAAGTACAAGGGGATA
	FL	CTTGGCATGATGCTGGAAGTGGC
	LC	ATGGTTCCACCAGTTGGCAGAGGC
ADCY3 Gene ID: 100514402	F	GCGATTACCTGGATGAGA	339
	R	CGTTGAGTAGCTGGTTGA
	TM	F-AACCTACCTCATCATTGCGTCCA-Q

F, R—primers (forward, reverse), FL and LC—FRET probes, TM—hydrolysis probe.