FIGURE 2.

Sequence alignment of C. fetus (Cf) PglX, PglY, and C. jejuni (Cj) PglH. Black boxes indicate specific amino acids associated with activity in PglH (Troutman and Imperiali, 2009; Ramirez et al., 2018). Black and dark gray amino acids represent functional residues that show non-conserved substitutions in PglX and PglY. Light gray highlighted sequences indicate EX₇E motifs commonly found in glycosyltransferases (Cid et al., 2000; Coutinho et al., 2003; Troutman and Imperiali, 2009). The catalytic EX₇E motif of Cj-PglH is located at residues E266 to E274. Cf-PglX CFF8240_1386, Cf-PglY CFF8240_1385, and Cj DDV78_00080 sequences were analyzed by Jalview (Waterhouse et al., 2009).