FIGURE 9.

Mutations in N-linked protein glycosylation decreased efflux in pglX- and pglJ- strains. (A) Accumulation of ethidium bromide in cultures of Cff WT (circle), pglJ- (square), and pglX- (triangle) over a time frame of 20 min. Relative fluorescent units (RFU) are indicated on the y-axis. (B) Bar graph depicting the relative fluorescence at t = 20 min. The error bars represent the standard error for each data set consisting of four biological replicates with three technical replicates each. ** p-value ≤ 0.001, * p-value ≤ 0.05 as determined by a two-tailed t-test.