Figure 1.

(A) A-to-I RNA editing is the result of a site selective deamination reaction catalyzed by ADAR enzymes. (B) A-to-I editing alters base pairing properties of the nucleobase, resulting in I:C hydrogen bonding. (C) Crystal structure of EndoV (PDB 2W35) bound to a single-stranded DNA substrate (purple) with inosine (red) and a Mg²⁺ ion (blue). (D) EndoV supplemented with Mg²⁺ results in cleavage of inosine containing transcripts, while Ca²⁺ promotes binding. (E) General concept of immunoprecipitation using E. coli EndoV (eEndoV) – maltose binding protein (MBP) fusion and anti-MBP magnetic beads.