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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Chem Biol. 2020 Jun;12(2):e82. doi: 10.1002/cpch.82

Figure 2.

Figure 2.

EndoVIPER-seq protocol workflow. mRNA starting material is 1) randomly hydrolyzed into 200–500 nt fragments followed by 2) glyoxal denaturation (turquoise hexagons). A-to-I edited transcripts are enriched with 3) EndoVIPER pulldown and 4) glyoxal adducts are removed prior to RNA-seq library preparation and sequencing.