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EndoVIPER-seq protocol workflow. mRNA starting material is 1) randomly hydrolyzed into 200–500 nt fragments followed by 2) glyoxal denaturation (turquoise hexagons). A-to-I edited transcripts are enriched with 3) EndoVIPER pulldown and 4) glyoxal adducts are removed prior to RNA-seq library preparation and sequencing.
