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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Chem Biol. 2020 Jun;12(2):e82. doi: 10.1002/cpch.82

Figure 3.

Figure 3.

(A) Glyoxal is used to covalently and reversibly denature RNA fragments. (B) Glyoxal reacts with the Watson-Crick-Franklin face of nucleobases, strongly inhibiting secondary structure and maintaining RNA in single-stranded conformation for optimal EndoVIPER pulldown.