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. Author manuscript; available in PMC: 2020 Jun 24.
Published in final edited form as: Eur Respir J. 2019 May 30;53(5):1801291. doi: 10.1183/13993003.01291-2018

Figure 3. Eosinophils directly modulate alveolar macrophage production of MMP-12 through IL-13 dependent mechanisms.

Figure 3.

ELISA measurement of MMP-12 in cell culture supernatant from in vitro 48h cultures of eosinophils (Eos) and/or alveolar macrophages (Macs). (A) Eosinophils cultured alone, macrophages cultured alone, and co-culture (Eos+Mac). Wild type eosinophils were activated (IL-33 for 24 hours then washed) and cultured with WT macrophages or MMP-12−/− macrophages showing the MMP-12 is from macrophages. (B) Assessment of co-culture supernatants from resting eosinophils (Eos) vs. activated eosinophils (Eos(A)) with macrophages (Macs) demonstrated that pre-activation of eosinophils (IL-33 for 24 hours then washed) enhances macrophage MMP-12 production. (C) Transwell co-culture of activated eosinophils with macrophages shows that contact is not required for the enhanced macrophage MMP-12 production. (D) ELISA measurements from co-culture supernatants of IL-13−/− vs. Wild Type (WT) eosinophils with WT macrophages demonstrates that eosinophil-derived IL-13 is promoting macrophage MMP-12 production. ***:p<0.001, ****:p<0.0001, ns: not significant.