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UMUC3 cells were transfected with MIR22HG vector or miR-486 mimic. Overexpression of MIR22HG and miR-486 was confirmed by qPCR at 48h post-transfection (A). The effects of MIR22HG overexpression on miR-486 (B) and the effects of miR-486 overexpression on MIR22HG (C) were explored by qPCR. Mean values of three independent replicates were presented. *, P<0.05.
