Figure 4. Competitive ELISA analysis for the specific binding of αRep9A8 and the short synthetic peptides.

The competitive reactions were reviewed by anti-His HPR and measured OD at 450 nm and the signals obtained from peptide coated well with no competitor in each set was attributed the 1-unit value. The relative binding activity was calculated using the following formula: [OD_{with competitor}/OD_{no competitor}] × 1. y-axis; the relative binding, x-axis; list of peptides coated wells (P4–P12). Data presented are from triplicate experiments (mean ± SD), analyzed using t test. *P<0.05 and **P<0.01.