Skip to main content
. 2020 Jun 23;40(6):BSR20201275. doi: 10.1042/BSR20201275

Figure 4. CircSLAMF6 acted as a sponge of miR-204-5p in GC cells.

Figure 4

(A) The binding sites between circSLAMF6 and miR-204-5p were predicted by circBank. (B–E) Dual-luciferase reporter assay and RIP assay were performed to verify the interaction between circSLAMF6 and miR-204-5p in AGS and MKN-45 cells. (F,G) Relative expression of miR-204-5p in AGS and MKN-45 cells transfected with si-NC, si-circSLAMF6, si-circSLAMF6 + anti-miR-NC, or si-circSLAMF6 + anti-miR-204-5p was examined by qRT-PCR. (H,I) Relative expression of miR-204-5p in GC tissues and cells was determined by qRT-PCR. (J) The correlation between circSLAMF6 and miR-204-5p expression in GC tissues was analyzed by Pearson correlation analysis. (K,L) The abundance of miR-204-5p in AGS and MKN-45 cells after hypoxia exposure for various times was detected by qRT-PCR. *P<0.05.