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. 2020 Jun 23;40(6):BSR20201275. doi: 10.1042/BSR20201275

Figure 5. Silencing circSLAMF6 impaired glycolysis, migration, and invasion in GC cells under hypoxia by targeting miR-204-5p.

Figure 5

AGS and MKN-45 cells were transfected with si-NC, si-circSLAMF6, si-circSLAMF6 + anti-miR-NC, or si-circSLAMF6 + anti-miR-204-5p after treatment of hypoxia. (A–D) Glucose consumption and lactate production were assessed by using the Glucose Assay Kit and Lactate Assay Kit, respectively. (E,F) The protein expression of HK2 was measured by Western blot. (G–J) Transwell assay was carried out to analyze cell migration and invasion. (K,L) The protein levels of MMP2 and MMP9 were examined via Western blot. *P<0.05.