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. 2020 Jun 23;40(6):BSR20201275. doi: 10.1042/BSR20201275

Figure 6. CircSLAMF6 could regulate the expression of MYH9 targeted by miR-204-5p.

Figure 6

(A) The binding sites between miR-204-5p and MYH9 were predicted using StarBase 3.0 database. (B–E) Dual-luciferase reporter assay and RIP assay were utilized to confirm the interaction between miR-204-5p and MYH9 in AGS and MKN-45 cells. (F,G) Both mRNA and protein levels of MYH9 in AGS and MKN-45 cells transfected with miR-NC or miR-204-5p were determined by qRT-PCR and Western blot, respectively. (H) MYH9 mRNA expression in GC tumor tissues and normal tissues was analyzed download from TCGA database. (I,J) The mRNA and protein levels of MYH9 in GC tissues and cells were assessed by qRT-PCR and Western blot, respectively. (K) The protein expression of MYH9 in GC cells (AGS and MKN-45) and normal cells (GES-1) was measured by Western blot. (L-N) The mRNA and protein levels of MYH9 in AGS and MKN-45 cells after hypoxia exposure for various times were evaluated by qRT-PCR and Western blot, respectively. (O,P) The protein expression of MYH9 in AGS and MKN-45 cells transfected with si-NC, si-circSLAMF6, si-circSLAMF6 + anti-miR-NC, or si-circSLAMF6 + anti-miR-204-5p was examined by Western blot. (Q,R) The correlation between MYH9 and miR-204-5p or circSLAMF6 expression in GC tissues was analyzed using Pearson correlation analysis. *P<0.05.