Serum estradiol and organ/tissue weights at necropsy in males without or with obstructed bladders and/or kidney abnormalities based on observational criteria (conducted blind to treatment group). (A) Serum estradiol concentrations in CTL, BPA, and DES animals with normal (N) or abnormal (ABN) bladder and/or kidneys. (B–E) Organ weights in the same animals (right testis, combined preputial glands, combined gonadal fat pads and right kidney). Values are mean ± SEM. ^ P = 0.07, * P < 0.05, ** P < 0.01, *** P < 0.001 vs. CTL-0-N; † P < 0.05 vs. the same perinatal treatment without T+E2 in adulthood.