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. 2020 Jun 5;21(11):4049. doi: 10.3390/ijms21114049

Figure 3.

Figure 3

MMTL enhances NTG-induced migraine-like hypersensitivity. (A) Timeline of this experiment. (B) MMTL pretreatment prolonged the higher dose (10 mg/kg) of NTG-decreased head withdrawal threshold in the periorbital region (n = 6–8 per group). Ftreatment (3, 24) = 61.580, p < 0.001. Ftime (11, 264) = 10.990, p < 0.001; Finteraction (33, 264) = 7.143, p < 0.001. (C) NTG (10 mg/kg) with or without MMTL pretreatment did not induce light-aversive behavior at 90 min or 24 h after NTG (n = 5–9 per group). (D,E) MMTL pretreatment enabled the lower dose (1 mg/kg) of NTG to decrease the head withdrawal threshold in the periorbital region (n = 6–8 per group; D), but did not induce the light-aversive behavior at 90 min or 24 h after NTG (n = 5–8 per group; E). Ftreatment (3, 22) = 22.660, p < 0.001; Ftime (10, 220) = 6.787, p < 0.001; Finteraction (30, 220) = 7.433, p < 0.001 for (D). *** p < 0.001, ** p < 0.01, * p < 0.05 vs. the corresponding time points in the “sham + vehicle” group (B,D). MMTL: masseter muscle tendon ligation; NTG: nitroglycerin; NTG(1): 1 mg/kg of nitroglycerin; NTG(10): 10 mg/kg of nitroglycerin.