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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Science. 2020 May 1;368(6490):497–505. doi: 10.1126/science.aay0267

Fig. 1. Three subsets of luminal cells identified by scRNA-seq of the intact mouse prostate.

Fig. 1.

(A) Single cell census of the intact prostate. tSNE of scRNA-seq profiles colored by unsupervised clustering of 15 subsets and labeled post hoc. (B) Prostatic luminal subtypes. tSNE of scRNA-seq profiles only from the luminal clusters in (A). (C) Validation of luminal subset markers in situ. IF staining of L1 (CD26/Dpp4, Cyan, top) and L2 (Tacstd2/Trop2, Red, middle) markers in the proximal and distal anterior lobe, along with Epcam (for epithelial cells, white), Ck5 (basal cells, green) and DAPI (nuclei). Bottom: IHC staining of Foxi1 in the proximal and distal anterior lobe. (D) Sharp transition from L2 to L1 cells. IF staining of L1 (CD133/Prom1 or CD26/Dpp4) and L2 (Tacst2/Trop2) markers, along with Epcam (for epithelial cells), Ck5 (basal cells) and DAPI (nuclei). A distinct border is observed between proximal and distal prostatic regions. Scale bars: 100 or 50 μm as labeled.