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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Toxicology. 2020 May 1;440:152473. doi: 10.1016/j.tox.2020.152473

Figure 1: Transgenic C. elegans expressing zebrafish CYP1A were created by microinjection.

Figure 1:

Bacterially-expressed zebrafish CYP1A shows strong EROD activity at 20° C (panel A). Transgenic worms express CYP1A mRNA as measured through mRNA average cycle threshold (Ct), while wild-type animals have a similar Ct to NTC (>36) despite similar expression of a housekeeping gene, cdc-42 (panel B). Transgenic worms also have robust CYP1A activity in isolated microsomes (panel C); microsomal EROD is undetectable in wild-type worm microsomes (panel C). Statistical significance was assessed by unpaired t-test. Asterisks represent statistical significance: *p<0.05, ****p<0.0001.