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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Toxicology. 2020 May 1;440:152473. doi: 10.1016/j.tox.2020.152473

Figure 4: CYP1A protects against BaP and ERSE-induced ATP reduction.

Figure 4:

The time frame for exposure and ATP determination is shown in panel A. One hundred larval stage 4 nematodes were dosed for 24 hours in BaP (panel B) or ERSE (panel D). Steady-state ATP levels were measured using a microplate reader. Fifty of the worms were allowed a 24 hour recovery before ATP measurement (panel C & E). Four technical replicates were run in individual wells and the experiment was repeated three times. Statistical significance was assessed by a two-way ANOVA followed by an unpaired t-test comparing all groups to each other. The two-way ANOVA did not find a statistically significant interaction between the effects of BaP exposure concentration and strain (p=0.13) immediately after exposure. Dose response across strains immediately after exposure was significant (main effect of dose p<0.0001).The two-way ANOVA showed a statistically significant interaction between the effects of ERSE exposure concentration and strain (p<0.0001) immediately after exposure. Dose response immediately after ERSE exposure across strains was significant (main effect of exposure, p<0.0001). Asterisks represent statistical significance: **p<0.01, ****p<0.0001.