Table 2. Transposon suppressor mutations of the P. aeruginosa PAO1 ΔfliC RSCV phenotype.
| Insertion site | # insertions | Function of disrupted gene1 | Operonic structure1 (class2) |
|---|---|---|---|
| Regulator of secondary metabolism (rsm) signalling pathway | |||
| gacS | 1 | global activator of cyanide synthesis, sensor histidine kinase, signal transduction | gacSldhA-PA0926-PA0925 |
| Flagellum biosynthesis | |||
| fleQ | 5 | transcriptional regulator | monocistronic (I) |
| fleN | 4 | flagellar synthesis regulator | flhFfleN (II) |
| motA | 4 | flagellum stator protein, exerts torque against motor switch | motAB |
| motB | 5 | flagellum stator protein, converts proton energy into torque | motAB |
| motC | 1 | flagellum stator protein, exerts torque against motor switch | PA1458-PA1459-motCD-PA1462 |
| PA1462 | 1 | unknown function | PA1458-PA1459-motCD-PA1462 |
| C-di-GMP signal transduction | |||
| sadB | 6 | unknown function | monocistronic |
| sadC | 2 | GDDEF domain protein | sadC-PA4331-PA4330 |
| siaA | 3 | hypothetical protein, predicted HAMP domain | siaABCD |
| siaB | 1 | hypothetical protein | siaABCD |
| siaD | 1 | GGDEF domain protein, diguanylate cyclase | siaABCD |
| Type IV pilus | |||
| fimX | 1 | type four pilus biosynthesis, GGDEF-EAL domain protein | fimWfimX |
| pilB | 2 | type four fimbrial biogenesis protein | monocistronic |
| pilC | 1 | type four fimbrial biogenesis protein | pilCDcoaE-PA4530 |
| pill | 1 | component of chemotactic signal transduction system, response regulator | pilGHI |
| pilM | 2 | type four fimbrial biogenesis assembly protein, ATPase | pilMNOPQ |
| pilO | 1 | type four pilus assembly, O-glycosyltransferase | pilMNOPQ |
| pilQ | 1 | type four fimbrial biogenesis outer membrane protein precursor | pilMNOPQ |
| pilR | 1 | two-component response regulator | pilSR |
| pilW | 2 | type four fimbrial biogenesis protein | fimUpilVWXY1Y2E |
| pilY1 | 4 | type four fimbrial biogenesis protein, tip associated adhesin | fimUpilVWXY1Y2E |
| Unknown function | |||
| PA1766 | 2 | hypothetical protein, predicted ATP-grasp domain | PA1768-PA1767-PA1766 |
| PA1767 | 4 | hypothetical protein, predicted cytoplasmic membrane protein | PA1768-PA1767-PA1766 |
| PA1768 | 1 | hypothetical protein, predicted type I export signal | PA1768-PA1767-PA1766 |
| PA1769 | 1 | hypothetical protein | monocistronic |
1Annotations and predicted operonic structures for flagellar genes were taken from Dasgupta and colleagues [45]. All other annotations and predicted operonic structures were retrieved from the Pseudomonas Genome Database [82] on April 5, 2020.
2Class as defined by the established four-tier flagellar gene transcriptional hierarchy in P. aeruginosa by Dasgupta and colleagues [45].