Table 3. Identity and frequency of RSCV-linked alleles in model biofilms.
| Reactor | Population size (1010 CFU/reactor) |
Est. RSCV frequency | RSCV-linked mutation(s)1 | Description | Est. allele frequency (a/n)2 |
|---|---|---|---|---|---|
| 1 | 10.54 ± 0.05 | 0.51 | fliG128T>G (V43G) | flagellum motor switch protein | 0.22 (15/34) |
| fliG834_844ΔGAAGGTCTTCA | flagellum motor switch protein | 0.12 (8/34) | |||
| 2 | 10.79 ± 0.03 | 0.55 | fliM718C>T (Q240*) | flagellum motor switch protein | 0.23 (40/94) |
| 3 | 10.53 ± 0.03 | 0.46 | wspF777C>A (S259R) | probable methylesterase | 0.01 (1/72) |
| 4 | 10.64 ± 0.08 | 0.60 | tpbB668A>G (D223G) | diguanylate cyclase | 0.01 (2/98) |
| fliH178G>T (E60*) | putative flagellum TTSS protein | 0.33 (54/98) | |||
| 5 | 10.47 ± 0.05 | 0.73 | fliM915delC | flagellum motor switch protein | 0.63 (49/57) |
1Nomenclature extensions to describe complex mutations adapted from Dunnen and Antonarkakis [83].
2n denotes the number of RSCVs isolated in a random sample from the reactor, and a denotes the number of those RSCVs that had the indicated RSCV-linked allele, which was determined by targeted sequencing of the allele in each isolate. Allele frequency in the biofilm population, therefore, was estimated as the ratio of a/n.