FIGURE 4.

Alignment of the region defined by residues 80–110 of IFABP and LFABP. Alignment was generated by Clustal Omega. ⁵⁰ Lines indicate a fully conserved residue, colon indicates residues with strongly similar properties, period indicates residues with weakly similar properties. Arrows indicate the alignment of Arg106 of IFABP, which is thought to be responsible for binding the carboxylate of the ligand, to Thr102 of LFABP which was shown to have a significant role in binding fatty acid. Strikingly, LFABP has five threonine residues within this region, whereas IFABP has only two. Threonines are indicated in red