TABLE 1.
Equilibrium dissociation constants (K d(LIGAND)) for ligands in this study a
| Fatty acids | Fatty acid amides | Glycerol esters | |
|---|---|---|---|
| ARA | AEA | 2‐AG | |
| IFABP | 4.3 ± 3.1 | 23.0 ± 2.7 | 183.8 ± 14.5 |
| LFABP | 18.9 ± 10.8 | 239.7 ± 46.6 | 475.4 ± 52.7 |
| OLA | OEA | 2‐OG | |
| IFABP | 0.9 ± 0.4 | 48.4 ± 11.4 | 160.4 ± 25.7 |
| LFABP | 7.7 ± 5.5 | 168.9 ± 27.1 | 333.9 ± 51.7 |
| PAL | PEA | 2‐PG | |
| IFABP | 3.3 ± 2.0 | 150.8 ± 16.6 | 106.0 ± 25.9 |
| LFABP | 21.6 ± 10.6 | 585.1 ± 9.7 | 1,536.5 ± 131.5 |
Note: In order to calculate K d(LIGAND), entire binding curves were performed in duplicate and then were repeated on at least two different days, for a minimum of four measurements. Calculation of K d(LIGAND) is dependent on mathematical curve fitting which can result in high values for SEM. The defined trends in affinity are within the limits of the reported error.
a
Values are given as μM ± SEM.