FIGURE 3.

PMA induces NETosis depending on the time and concentration of exposure. Fresh purified neutrophils were exposed to PMA for different times (30 minutes, 1, 2, and 3 hours), A, at different concentrations (10, 20, and 40 nM), B. Six single images were taken on each slide and the three different markers were quantified with ImageJ. *Statistically different from the untreated control P < .05. Biological replicates (independent experiments): n = 3 for 30 minutes time point; n = 2 for 1 and 2 hours time points; n = 7 for 3 hours; n = 5 for the dose effect experiment. Error bars depict SD