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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Dev Biol. 2020 Mar 19;462(2):197–207. doi: 10.1016/j.ydbio.2020.03.011

Figure 4. Loss of RB1 reduces photoreceptor, ganglion and bipolar layers.

Figure 4.

(A) Representative wild-type and RB1−/− organoids at D20, 30, 60 and 90 sectioned and immunostained against Chx10 and DAPI. (B) Representative wild-type and RB1−/− organoids at D60 and 90 sectioned and immunostained against Elavl3/4 and Pax6; Crx; Recoverin and Neurod; counterstained with DAPI. (C) Representative wild-type and RB1−/− organoids at D60 and 90 sectioned and immunostained against OTX2 and counterstained with DAPI. (D) The graphs show the percentage of Chx10+/DAPI+ area at different time points. (E) The percentage of Elavl3/4+, Pax6+, Crx+, Recoverin+, Neurod+ and OTX2+ cells out of the DAPI+ area at D60 and 90. The results are mean±s.e.m. of 9 organoids from three independent experiments (*P<0.05, **P<0.01, Student’s t-test). (F) Relative mRNA levels of retinal genes measured by qRT-PCR. The results are mean±s.e.m. of 3 organoids from three independent experiments (*P<0.05, **P<0.01, Student’s t-test). Scale bars: 50 μm.