Fig. 2. dsRNA levels, protein expression, and immune activation from hEPO mRNA incorporating uridine or 1mΨ, made by processes A or B, with or without RNase III treatment.

(A) dsRNA content was determined by dsRNA ELISA. (B) hEPO expression and (C) IFNβ response were measured by Ella in BJ fibroblasts at 48 hours after transfection. Data represent two independent experiments, with n = 2 to 3 technical replicates.