| Reagents | Volume (μL) | Final concentration |
|---|---|---|
| H2O | 1660 | |
| 10× DNA ligase reaction buffer | 200 | 1× |
| 10 % (vol/vol) Triton X-100 | 20 | 0.1% (vol/vol) |
| BSA (20 mg/mL) | 100 | 1 mg/mL |
| T4 DNA ligase (2000 U/μL) | 4 | 4 U/μL |
| RNasinPlus (40 U/μL) | 16 | 0.5 U/μL |
| Total volume | 2000 |
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