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. 2020 Jun 24;6(26):eaba0154. doi: 10.1126/sciadv.aba0154

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

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Fig. 2 — (A) Experimental design. (B) Scheme illustrating the arrangement of electrodes in a typical experiment. (C) Representative diffusion curves and their parameters obtained in vivo in the cortical layer V of a control and an alcohol-exposed rat or in the diluted agar. (D) Quantification of volume fraction (α) in control (white) and alcohol-exposed (black) msP rats. A slightly significant effect of alcohol was found by two-way ANOVA [F(1,38) = 6.7, P < 0.05]. No effects of cortical layer or interaction between alcohol treatment and cortical layer were found. (E) Quantification of tortuosity (λ) in control (white) and alcohol-exposed (black) msP rats. A robust significant effect of alcohol was found by two-way ANOVA [F(1,38) = 21.6, P < 0.0001]. No effects of cortical layer or interaction were found. For all panels, *P < 0.05 and ***P < 0.001.