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. 2020 Jun 24;6(26):eaba4221. doi: 10.1126/sciadv.aba4221

Fig. 1. Identification of 16 transcriptionally distinct cell populations in the adult rat NAc.

Fig. 1

(A) snRNA-seq workflow. Male and female adult rats (n = 4 per sex per treatment) received saline or cocaine (20 mg/kg) (intraperitoneal injection) and underwent locomotor testing before tissue harvesting, NAc dissection (tissue punch area highlighted in blue), nuclei purification, and single-nucleus sequencing on the 10x Genomics platform. (B) Global clustering across experimental treatment and sex for 15,631 individual NAc nuclei identifies all major cell classes of the rat NAc, including MSNs expressing Drd1 and Drd2 mRNA. UMAP, Uniform Manifold Approximation and Projection. (C and D) Enrichment of Drd1 and Drd2 within identified cell types. (E) Dot plot indicating the average expression and percent of cells expressing marker genes of each identified cell type. GABA, γ-aminobutyric acid. (F) Heat map of cell-specific marker genes across all clusters.