Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene Mus musculus | zinc finger, CW type with PWWP domain 1(Zcwpw1) | Mouse Genome Informatics | MGI:2685899 | |
| Gene Mus musculus | PR domain containing 9 (Prdm9) | Mouse Genome Informatics | MGI:2384854 | |
| Strain, strain background Mus musculus C57BL6/J) | Mouse: C57BL/6J | Jackson laboratory | RRID:IMSR_JAX:000664 | All sexes used |
| Genetic reagent Mus musculus C57BL6/J) | Zcwpw1−/− | PMID:31453335 | Materials and methods section Generation of Zcwpw1 knockout mice |
|
| Genetic reagent Mus musculus C57BL6/J) | Zcwpw1KI/KI | This study | Materials and methods section Mice |
|
| Genetic reagent Mus musculus C57BL6/J) | Prdm9−/− | This study | Materials and methods section Mice |
|
| Antibody | Rabbit polyclonal anti-ZCWPW1 | PMID:31453335 | IF 1:1000; WB 1:5000; ChIP 35 µg | |
| Antibody | Rabbit polyclonal anti-alpha Tubulin | Proteintech Group | 11224–1-AP RRID:AB_2210206 |
WB 1:10000 |
| Antibody | Rat polyclonal anti-ZCWPW1 | This study | Materials and methods section IF 1:200 |
|
| Antibody | Mouse polyclonal anti-SCP3 | Abcam | #ab97672 RRID:AB_10678841 |
IF 1:500 |
| Antibody | Rabbit polyclonal anti-SCP1 | Abcam | # ab15090 RRID:AB_301636 |
IF 1:2000 |
| Antibody | Rabbit polyclonal anti-RAD51 | Thermo Fisher Scientific | #PA5-27195 RRID:AB_2544671 |
IF 1:200 |
| Antibody | Rabbit polyclonal anti-DMC1 | Santa Cruz Biotechnology | #sc-22768 RRID:AB_2277191 |
IF 1:100 |
| Antibody | Mouse polyclonal anti-γH2AX | Millipore | #05–636 RRID:AB_309864 |
IF 1:300 |
| Antibody | Mouse polyclonal anti-pATM | Sigma-Aldrich | #05–740 RRID:AB_309954 |
IF 1:500 |
| Antibody | Rabbit polyclonal anti-MSH4 | Abcam | #ab58666 RRID:AB_881394 |
IF 1:500 |
| Antibody | Rabbit polyclonal anti-RNF212 | this study | a gift from Mengcheng Luo, Wuhan University | Materials and methods section IF 1:500 |
| Antibody | mouse polyclonal anti-MLH1 | BD Biosciences | #550838 RRID:AB_2297859 |
IF 1:50 |
| Antibody | Rabbit polyclonal anti-H3K4me3 | Abcam | #ab8580 RRID:AB_306649 |
IF 1:500 ChIP 5 µg |
| Antibody | Rabbit polyclonal anti-H3K36me3 | Abcam | #ab9050 RRID:AB_30696 |
IF 1:500 ChIP 5 µg |
| Antibody | Goat anti-rabbit IgG (H+L) cross-adsorbed secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | #A-11070 RRID:AB_142134 |
IF 1:500 |
| Antibody | Goat anti-rabbit IgG H and L (Alexa Fluor 594) preadsorbed | Abcam | ab150084 RRID:AB_2734147 |
IF 1:500 |
| Antibody | Goat anti-mouse IgG H and L (Alexa Fluor488) | Abcam | ab150113 RRID:AB_2576208 |
IF 1:500 |
| Antibody | Goat anti-mouse IgG H and L (Alexa Fluor 594) preadsorbed | Abcam | ab150120 RRID:AB_2631447 |
IF 1:500 |
| Antibody | Goat anti-rat IgG H and L (Alexa Fluor 488) preadsorbed | Abcam | ab150165 RRID:AB_2650997 |
IF 1:500 |
| Sequence-based reagent | Primers used in this study | This study | All primers used in this study are shown in the methods | |
| Commercial assay or kit | NEBNext Ultra II DNA Library Prep Kit | NEB | E7645S | |
| Software, algorithm | Trimmomatic v0.32 | doi:10.1093/bioinformatics/btu170 | http://www.usadellab.org/cms/index.php?page=trimmomatic | |
| Software, algorithm | Bowtie2 v2.3.4.2 | doi:10.1038/nmeth.1923 | RRID:SCR_005476 | |
| Software, algorithm | Samtools and Picard | doi:10.1038/ng.806 | http://samtools.sourceforge.net | |
| Software, algorithm | MACS2 v2.1.0 | doi:10.1186/gb-2008-9-9-r137 | RRID:SCR_013291 | |
| Software, algorithm | Integrative Genomics Viewer | doi:10.1038/nbt.1754 | RRID:SCR_011793 | |
| Software, algorithm | Deeptools2 | doi:10.1093/nar/gkw257 | http://deeptools.ie-freiburg.mpg.de | |
| Software, algorithm | HOMER software | doi: 10.1016/j.molcel.2010.05.004 | RRID:SCR_010881 | |
| Software, algorithm | GREATER software | doi:10.1038/nbt.1630 | http://great.stanford.edu | |
| Software, algorithm | regioneR package version1.18.1 | doi: 10.1093/bioinformatics/btv562 | http://www.bioconductor.org/packages/regioneR | |
| Software, algorithm | hisat2 v2.1.0 | doi: 10.1038/nprot.2016.095 | http://github.com/infphilo/hisat2 | |
| Software, algorithm | DESeq2 v 1.18.0 | doi: 10.1186/s13059-014-0550-8 | RRID:SCR_015687 | |
| Software, algorithm | Metacape | doi: 10.1038/s41467-019-09234-6 | http://metascape.org |