Figure 3. BLOSUM62 cluster map of NPY/NPF/PrPR/sNPF-type receptors and closely related tachykinin-type receptors (TKR) and luqin/RYamide-type receptors (LQ/RYaR).

Nodes are labelled with phylum-specific colours, as shown in the key, and connections represent BLAST relationships with a P value > 1e-65. Note that the echinoderm receptors (boxed) have more connections with PrRP/sNPF-type receptors than with NPY/NPF-type receptors. The sequences of the receptors included in this figure are listed in Figure 3—source data 1.

Figure 3—figure supplement 1. Asterias rubens sNPF/PrRP-type receptor (Ar-sNPF/PrRPR) transcript.

Sequence of a transcript (contig 1120879 from A. rubens radial nerve cord transcriptome) that encodes a sNPF/PrRP-type receptor. The transcript sequence (lowercase; numbering on the left) and the deduced amino acid sequence (uppercase and highlighted in grey; numbering on the right) are shown. A cDNA encoding the open reading frame was cloned by PCR using primers corresponding to the sequences highlighted in yellow, ligated into the vector pCDNA 3.1 (+) and sequenced. The sequence of the cloned cDNA was identical to the open reading frame of contig 1120879, with the exception of a single nucleotide substitution (t to c; highlighted in black), which results in replacement of an isoleucine residue (codon: ata in contig 1120879) with a threonine residue (codon: aca in the cloned cDNA). This sequence has been deposited in GenBank under the accession number MH807444.1.