Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
APOE3-TR+/+
APOE4-TR+/+ |
PMID:8980023 | ||
| Strain, strain background (M. musculus) | C57BL/6J (B6) | Jackson laboratory | 000664; RRID:IMSR_JAX:000664 | |
| Cell line (M. musculus) | BV2 microglia | ATCC | EOC 20 (ATCC CRL-2469); RRID:CVCL_5745 | Female originated |
| Other | Mixed glia (microglia and astrocyte) | R. norvegicus | Postnatal days 3–5, mixed sexes | |
| Transfected construct (M. musculus) | Nfe2l2 siRNA | Thermofisher Scientific | 156499 | |
| Other | Lipofectamine RNAiMAX reagent | Thermofisher Scientific | 13778500 | |
| Antibody | anti-NRF2 (rabbit polyclonal) | Abcam | ab137550; RRID:AB_2687540 | WB, 1:1000 |
| Antibody | anti-H3 (rabbit polyclonal) | Cell Signaling Technology | D1H2; RRID:AB_10544537 | WB, 1:1000 |
| Antibody | anti-GAPDH (Mouse monoclonal) | Santa Cruz Biotechnology | sc-32233; RRID:AB_627679 | WB, 1:500 |
| Antibody | anti-NFKBP65 (Rabbit polyclonal) | Cell Signaling Technology | D14E12; RRID:AB_10859369 | WB, 1:750 |
| Antibody | anti-mouse IRDye 800CW | LICOR | 926–32210; RRID:AB_621842 | WB, 1:20,000 |
| Antibody | anti-rabbit IRDye 680RD | LICOR | 926–68070; RRID:AB_10956588 | WB, 1:20,000 |
| Commercial assay or kit | RNAeasy Mini Kit | Qiagen | 74104 | |
| Commercial assay or kit | TRUseq Stranded mRNA Kit | Illumina | 20020594 | |
| Commercial assay or kit | qScript cDNA Supermix | Quantabio | ||
| Commercial assay or kit | Taq master mix | Biopioneer | MAT-2.1–10 | |
| Commercial assay or kit | 12–230 kDa Jess or Wes Separation Module | Protein Simple | SM-W004 | |
| Commercial assay or kit | V-PLEX proinflammatory panel one immunoassay | Mesoscale Diagnostics, Rockville, MD | K15048D | |
| Commercial assay or kit | V-PLEX Aβ Peptide Panel 1 (4G8) Kit | Mesoscale Diagnostics, Rockville, MD | K15199E | |
| Chemical compound, drug | TRIzol | Invitrogen | 15596026 | |
| Software, algorithm | Rstudio | Packages: LIMMA, WGCNA, BRETIGEA | ||
| Software, algorithm | Ingenuity pathway analysis | Qiagen | ||
| Software, algorithm | GraphPad Prism | Version 8 |