KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Experimental Models: Organisms/Strains | ||
| w;Pdf(BMRJ)-Gal4; | P. Taghert, Wash U Med. School | [15, 55] |
| w;;Pdf01 | P. Taghert, Wash U Med. School | [15, 55] |
| yw;UAS-hid/CyO | P. Taghert, Wash U Med. School | RRID:BDSC_65403 |
| w[1118];P(w[+mC]=Clk-lexA.4.1) 3/TM6C, Sb[1] | Amita Sehgal, U Penn | RRID:BDSC_80704 |
| w;UAS-CD8:GFP; | Bloomington Stock left | RRID:BDSC_5130 |
| w;;UAS-Dicer-2 | Bloomington Stock left | RRID:BDSC_24651 |
| yw;Pdf-LexA; | M. Rosbash, Brandeis | N/A |
| ;UAS-GluClαRNAi; | Vienna Drosophila Resource left | ID 105754 |
| w;UAS-Fas2; | Dr. V. Budnik, UMaSS Med. School | N/A |
| w;;UAS-Unc5-HA | Barry Dickson, Janelia Farm | [28] |
| UAS-Dscam-TM1-GFP; Pin/CyO; | Bing Ye, University of Michigan | [58] |
| ;;UAS-DbtLONG myc(27MIC)/(TM3) | Jeffrey Price, University of Missouri at Kansas City | [59] |
| w;;20xUAS-GCamp6f | Bloomington Stock left | RRID:BDSC_52869 |
| w;LexAop-mCD8GFP;TM2/TM6B,Tb | Bloomington Stock left | RRID:BDSC_66545 |
| Uas-mGluR-RNAi; | Vienna Drosophila Resource left | ID 103736 |
| ;UAS-GluClαRNAi; | Vienna Drosophila Resource left | ID 107971 |
| w;;UAS-mGluR-RNAi | Vienna Drosophila Resource left | ID 1793 |
| Antibodies | ||
| Mouse anti-PDF (1:500) | Developmental Hybridoma Bank | DSHB Cat# DPDF-P (nb33), RRID:AB_10805428 |
| Guinea pig anti- PAP (1:500) | Paul Taghert Lab | N/A |
| Rabbit anti-GFP (1:1000) | Invitrogen A-6455 | (Molecular Probes Cat# A-6455, RRID:AB_221570) Comments: Discontinued; This product offered by Molecular Probes (Invitrogen A-6455) |
| Software | ||
| Fiji | http://fiji.sc | RRID: SCR_002285 |
| Imaris | Bitplane | RRID:SCR_007370 |
| Matlab R2018a | MathWorks | RRID: SCR_001622 |
| GraphPad Prism 8.0 | GraphPad Software | RRID: SCR_002798 |
| R | The R Foundation | RRID:SCR_001905 |
| DAM FileScan | Trikinetics | [65] |
| ClockLab | Actimetrics | RRID:SCR_014309 |
| R package for Circular Statistics | Dr. Boyan Kostadinov, code developed for this study. | The code generated during this study is available at: https://github.com/bkostadi/circular_analysis |
| Chemicals, Peptides, and Recombinant Proteins | ||
| HardSet Vectashield Mounting Medium | Vector Laboratories | RRID:AB_2336787 |
| L-Glutamic Acid | Sigma-Aldrich | PubChem ID: 24895052 |
| Carbachol Chloride (CCH) | Tocris, Bristol, U.K. | PubChem ID: 5831 |
| Tetrodotoxin citrate (TTX) | Tocris, Bristol, U.K. | PubChem ID: 16759596 |
| Premix PBS Buffer (10x) | Sigma-Aldrich | Cat# 11666789001 |
| Triton X-100 | Sigma-Aldrich | Cat#X100; CAS: 9002-93-1 |
| Bacto Agar | Becton Dickinson Microbiology | Cat#214010 |
| Sucrose | Sigma-Aldrich | Cat#S7903; CAS: 57-50-1 |
| Other | ||
| DAM2 Drosophila Activity Monitors | Trikinetics | [65] |
| DAM Drosophila Environmental Monitors | Trikinetics | [65] |