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. 2020 Jun 8;39(26):4983–5000. doi: 10.1038/s41388-020-1349-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Venn diagrams present potential target genes of miR-92b-3p in three databases. Among the genes overlapped in the three databases, PTEN was the highest confidence target for miR-92b-3p. b A schematic drawing showing the possible binding sites of miR-92b-3p within the PTEN 3′-UTR and the corresponding site-specific mutations. c Relative reporter gene activity of vector containing PTEN 3′-UTR in NOZ and GBC-SD cells co-transfected with increasing amounts (10, 20, and 50 pmol) of miR-92b-3p mimic. Relative reporter gene activity of vector with PTEN 3′-UTR or mutant counterparts in NOZ and GBC-SD cells in the presence of 20 pmol miR-92b-3p mimic. d Stable overexpression or knockdown of miR-92b-3p affects the levels of PTEN mRNA. Data shown are means ± SD from three representative independent experiments. e RNA levels of miR-92b-3p and PTEN in immunoprecipitates are shown as fold enrichment in AGo2 relative to those in IgG. Data shown are means ± SD from three representative independent experiments. f PI3K/AKT signaling in NOZ cells stably knockdown of miR-92b-3p. g Immunoblotting analysis for PTEN protein level, as well as AKT (Ser473), 70S6K (Thr389), and eIF4EBP1 (phospho T37) phosphorylation in NOZ and GBC-SD cells stably overexpressing or knockdown of miR-92b-3p. h DCA affects the expression of PTEN mRNA in NOZ and GBC-SD cells. Data shown are means ± SD from three representative independent experiments. i Immunoblotting analysis for PTEN protein level, as well as AKT (Ser473), 70S6K (Thr389), and eIF4EBP1 (phospho T37) phosphorylation in NOZ and GBC-SD cells treated with DCA.