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. 2020 Jun 24;10:10241. doi: 10.1038/s41598-020-66857-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agarose gel electrophoresis of DNA extracts from BCC9546. (a) Genomic DNA running on 1% agarose gel: M, 250 ng DNA size marker (GeneRuler DNA Ladder Mix, Thermo Scientific); 1–2, 250 ng BCC9546 genomic DNA extract. (b) Plasmid DNA running on 0.5% agarose gel: Ms, 250 ng supercoiled DNA Ladder (New England BioLabs); p1, 50 ng BCC9546 plasmid DNA extract; p2, 120 ng BCC9546 plasmid DNA extract. The gel images were cropped for concise visualization. See Supplementary Fig. S1 for the uncropped gel images.