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. 2020 Jun 24;10:10297. doi: 10.1038/s41598-020-67346-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of IFN-γ and IL-10 produced from whole blood samples of β-thalassaemia patients upon stimulation. Whole blood samples (adjusted the number of lymphocyte plus monocyte at 1.8 × 10⁵ cells) from healthy controls (open circle, n = 13), β-thalassaemia patients (filled circle, n = 15) and diabetes individuals (filled triangle, n = 10) were cultured with medium alone, 10 μg/ml of LPS, 5.4 × 10⁶ CFUs PFA fixed Bp or 10 μg/ml FlgK protein for 48 h. IFN-γ (A) and IL-10 (B) production upon stimulation in supernatant were measured by ELISA, and the results were shown as scattered dot plot and line at mean with 95% confidence interval. Statistically significant was analyzed by using one-way ANOVA with Tukey's multiple comparisons post-test; *P < 0.05, **P < 0.01, ***P < 0.001, and ns non-significant.