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. 2020 Jun 24;10:10297. doi: 10.1038/s41598-020-67346-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of plasma anti-Bp IgG antibody level and IFN-γ production upon TCR stimulation between samples from healthy donors and β-thalassaemia patients. Whole blood from healthy control (open circle, n = 9) and β-thalassaemia patients (filled circle, n = 7) were stimulated with anti-CD3 and anti-CD28 antibodies for 48 h before measured for IFN-γ in supernatant by ELISA (A). Plasma anti-Bp IgG from healthy controls (open circle, n = 34) and β-thalassaemia patients (filled circle, n = 16) were measured by ELISA (B). The results were shown as scattered dot plot and line at median with interquartile range. Statistical analysis was done by using Mann Whitney test; *P < 0.05, ns non-significant.