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. 2020 Jun 24;10:10297. doi: 10.1038/s41598-020-67346-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increasing of whole blood HO-1 expression from β-thalassaemia patients compared to healthy donors with and without fixed Bp stimulation. HO-1 expression from whole blood of healthy controls (open circle, n = 7) and β-thalassaemia patients (filled circle, n = 7) were analyzed by real-time PCR using GADPH as internal reference gene. Unstimulated whole blood of healthy and β-thalassaemia were analyzed for ΔCt between HO-1 and GADPH before calculated for HO-1 mRNA expression by 2^−ΔCt. PFA fixed Bp stimulated whole blood of healthy and β-thalassaemia were analyzed for ΔCt between HO-1 and GADPH, then ΔΔCt comparing to medium control before calculated for HO-1 mRNA expression by 2^−ΔΔCt. Results were plotted as scattered dot plot and line at median with interquartile range. Statistical analysis was done by using Wilcoxon matched pairs signed rank test or Mann Whitney test; *P < 0.05 and **P < 0.01.