Figure 6.
Bacterial killing efficacies of human monocyte are related to inhibition or induction of HO-1 expression. Monocyte from healthy donors (n = 3) were pre-treated 3 h with medium, hemin at 25 μM (A), CoPP at 50 μM (B) or SnPP at 10 μM (C) before infection with 5 × 105 CFUs live Bp for 1, 3 and 6 h or infection with 5 × 105 CFUs of live E. coli, S. typhimurium or S. aureus for 0.5, 1 and 3 h. Total numbers of viable bacteria after infection at various time points were assessed by colony count, calculated as % bacterial killing = ((inoculum bacteria − remaining bacteria)/inoculum bacteria) × 100. Data is shown as individual dot plots with connecting lines of the same donors. Statistical analysis was done by using two-way ANOVA with Bonferroni's multiple comparisons test; *P < 0.05, **P < 0.01, ***P < 0.001.